Thermo Fisher Scientific ZMIZ1 Polyclonal Antibody
ZMIZ1 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot 및 IHC(P)에서 사용 가능. 인간과 생쥐 시료에 반응하며 항원 친화 크로마토그래피로 정제됨. PBS/glycerol buffer에 보관되며 -20°C에서 안정적. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:25–1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to residues near the N-terminal of human zinc finger, MIZ-type containing 1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2.8 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 40% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2636194 |
Product Specific Information
The antibody detects endogenous levels of total ZMIZ1 protein.
Target Information
Retinoic acid plays a critical role in development, cellular growth, and differentiation, and induces the expression of a variety of genes. RAI17 expression is induced by retinoic acid and is predominantly expressed in heart, brain, and ovaries. Within the brain, highest expression is in the amygdala. The deduced 1,067-amino acid protein contains an MSX-interacting zinc finger (MIZ) domain, a nuclear localization signal sequence, and two proline-rich regions. A strong intrinsic transactivation domain is identified in the C-terminal proline-rich region. RAI17 expression is detected in various cancer cell lines. RAI17 colocalizes with endogenous androgen receptor (AR) in the nuclei of prostate epithelial cells from human tissue samples. In human prostate cancer cells, RAI17 increases the transcriptional activity of AR. Studies using sumoylation-deficient AR mutants suggest that the increase of AR activity by RAI17 is dependent upon receptor sumoylation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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