Thermo Fisher Scientific Donkey anti-Rabbit IgG (H+L) Secondary Antibody, Qdot 565
Thermo Fisher Scientific의 Donkey anti-Rabbit IgG (H+L) Qdot 565는 토끼 IgG 검출용 형광 보조 항체로, WB, IHC, ICC, Flow Cytometry에 적합합니다. Qdot 565 형광체로 높은 밝기와 내광표백성을 제공합니다. 연구용으로만 사용됩니다.
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Applications and Tested Dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | 1:50–1:500 |
| Immunohistochemistry (IHC) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:50 |
| Flow Cytometry (Flow) | 1:50 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Rabbit |
| Host / Isotype | Donkey / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | Qdot™ 565 |
| Excitation / Emission Max | 300 / 564 nm |
| Form | Liquid |
| Concentration | 1 µM |
| Purification | Purified |
| Storage Buffer | 0.05M borate, pH 8.3, with 1M betaine |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient |
| RRID | AB_2556484 |
| Target | IgG |
| Antibody Form | Whole Antibody |
Additional Formats Available
- Alexa Fluor 488
- Alexa Fluor 594
- AP
- Biotin
- FITC
- HRP
- Qdot 525
- Qdot 625
- Qdot 655
- TRITC
- Custom conjugation available upon request
Product Specific Information
Qdot™ nanocrystals are semiconductor-based fluorescent particles that are exceptionally bright and resistant to photobleaching. When paired with appropriate optical filters, Qdot nanocrystals can be up to 50 times brighter than traditional organic dyes.
Target Information
Anti-Rabbit secondary antibodies are affinity-purified and exhibit high specificity for rabbit immunoglobulins. They are widely used for detection, sorting, or purification of target proteins. Secondary antibodies enhance assay versatility and sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody. These antibodies are typically produced by immunizing the host with pooled immunoglobulins from the target species and may be further purified or modified (e.g., immunoaffinity chromatography, fragmentation, label conjugation) to achieve high specificity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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