Thermo Fisher Scientific MICA/B Monoclonal Antibody (6D4), Biotin
인간 MICA 및 MICB 단백질을 인식하는 단클론 항체로, CD314(NKG2D) 활성 차단 가능. Biotin으로 표지되어 ICC/IF, Flow Cytometry, IP 등 다양한 면역분석에 사용. 고순도 크로마토그래피로 정제된 액상 형태.
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Applications
| Application | Tested Dilution |
|---|---|
| Immunocytochemistry (ICC/IF) | Assay-dependent |
| Flow Cytometry (Flow) | 1–12 µg/mL |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 6D4 |
| Immunogen | Transfected C1R cells expressing MICA |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Size-exclusion chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 15 mM sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2898638 |
Available Formats
- Alexa Fluor 647 ([MA5-38723])
- Alexa Fluor 700 ([MA5-38724])
- APC ([MA5-38725])
- FITC ([MA5-38727])
- PE-Cyanine7 ([MA5-38728])
- Request custom conjugation (Thermo Fisher form)
Product Specific Information
This antibody recognizes a common extracellular epitope on MICA and MICB glycoproteins, transmembrane ligands of CD314 (NKG2D), and is able to block CD314-mediated activation of NK cells and cytotoxic T cells.
Target Information
MICA encodes the highly polymorphic MHC (HLA) class I chain-related gene A. The protein product is expressed on the cell surface, although unlike canonical class I molecules, it does not associate with beta-2-microglobulin. MICA functions as a stress-induced antigen broadly recognized by intestinal epithelial gamma delta T cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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