Thermo Fisher Scientific Erk1, Erk2 Polyclonal Antibody
ERK1/2 단백질 인산화 경로 연구용 Rabbit Polyclonal 항체. Western blot 및 IHC에 적합하며 Human, Mouse, Rat에서 반응. 동결건조 형태로 제공되며 장기 보관 시 -20°C에서 안정적. 다양한 종에서 높은 상동성을 보임.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:300–1:2,000 | - |
| Immunohistochemistry (IHC) | 1:300–1:2,000 | - |
| Miscellaneous (PubMed) | - | View 3 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / Ig |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide from amino acid region 160–220 of the internal region of human Erk1, Erk2 (Mitogen-activated protein kinase 1 & 3, MAP kinase 1 & 2) conjugated to an immunogenic carrier protein. |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | Not Determined |
| Storage Buffer | Whole serum |
| Contains | No preservative |
| Storage Conditions | Store at 4°C for short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for stability. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Reconstitute in 100 µL of sterile water.
- Centrifuge to remove any insoluble material.
- The peptide shows homology across multiple species including rat, mouse, zebrafish, xenopus, and chicken.
- Specificity: Erk1 and Erk2.
Target Information
ERK1 and ERK2 are widely expressed kinases involved in the regulation of meiosis, mitosis, and postmitotic cellular functions.
These kinases are activated by various stimuli such as growth factors, cytokines, viral infection, and G protein-coupled receptor ligands.
Upon activation, the Ras-Raf-MEK-ERK signaling cascade leads to phosphorylation of ERK1/2, which subsequently targets cytoplasmic and nuclear substrates including transcription factors such as c-Fos and Elk-1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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