
Thermo Fisher Scientific EphA1 (extracellular) Polyclonal Antibody
EphA1 단백질의 세포외 영역을 인식하는 Rabbit Polyclonal Antibody로, Human, Mouse, Rat에 반응합니다. WB, IHC(F), ICC/IF, Flow Cytometry에 사용 가능하며, 항원 친화 크로마토그래피로 정제되었습니다. 연구용으로만 사용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:300 |
| Immunocytochemistry (ICC/IF) | 1:25 |
| Flow Cytometry (Flow) | 2.5 µg |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | (C)KKEPRQLELTWAGSR, corresponding to amino acid residues 457–471 of human EphA1, Extracellular, N-terminus |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.8 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Reconstitution: 25 µL, 50 µL, or 0.2 mL double distilled water (DDW), depending on the sample size.
The antibody ships as a lyophilized powder at room temperature. Upon arrival, store at -20°C.
The reconstituted solution can be stored at 4°C for up to 1 week. For longer storage, aliquot and keep at -20°C.
Avoid multiple freeze/thaw cycles. Centrifuge all antibody preparations before use (10,000 × g, 5 min).
Target Information
EphA1 belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family.
EPH and EPH-related receptors are involved in developmental events, especially in the nervous system.
Receptors in this subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and two fibronectin type III repeats.
Ephrin receptors are divided into two groups based on extracellular domain sequence similarity and ligand affinity (ephrin-A or ephrin-B).
EphA1 is expressed in some human cancer cell lines and has been implicated in carcinogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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