Thermo Fisher Scientific ZO-1 Polyclonal Antibody
ZO-1 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB, IHC, ICC, ELISA 등 다양한 응용에 사용 가능. Tight junction 관련 단백질 연구에 적합하며, 고순도 항원 친화 크로마토그래피로 정제됨. PBS buffer, 0.25 mg/mL 농도, -20°C 보관.
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Thermo Fisher Scientific ZO-1 Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.01–2 µg/mL | 256 |
| Immunohistochemistry (IHC) | 2–5 µg/mL | 249 |
| Immunohistochemistry (Paraffin) (IHC (P)) | – | 40 |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | – | 3 |
| Immunohistochemistry (Frozen) (IHC (F)) | – | 18 |
| Immunohistochemistry - Free Floating (IHC (Free)) | – | 2 |
| Immunocytochemistry (ICC/IF) | 1–10 µg/mL | 304 |
| Flow Cytometry (Flow) | – | 2 |
| ELISA | Assay-dependent | 4 |
| Immunoprecipitation (IP) | Assay-dependent | 13 |
| ChIP assay (ChIP) | – | 1 |
| Miscellaneous (PubMed) | – | 38 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Guinea pig, Human, Mouse, Non-human primate, Rat |
| Published Species | Avian, Bacteria, Bovine, C. elegans, Cat, Chicken, Cynomolgus monkey, Dog, Fish, Fruit fly, Hamster, Horse, Human, Insect, Mouse, Non-human primate, Pig, Plant, Rabbit, Rat, Rhesus monkey, Tag, Virus, Xenopus, Zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | 69 kD fusion protein corresponding to amino acids 463–1109 of human ZO-1 cDNA. This sequence lies N-terminal to the 80 amino acid region (alpha-motif) present in the a+-isoform but absent in the a–isoform due to alternative splicing. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.25 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.1% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2533938 |
Product Specific Information
- Catalog No. 61-7300 has been successfully used in Western blot, Immunoprecipitation, Immunofluorescence, ELISA, and Immunohistochemistry.
- For formalin-fixed, paraffin-embedded tissue sections, protease Type XIV (Actinase E) pretreatment is required (1–2 mg/mL at 37°C for 10 min).
- Other enzyme and heat-induced epitope retrieval methods may not be suitable.
- Frozen sections can be successfully stained after fixation with acetone at 4°C.
Target Information
ZO-1 forms complexes with ZO-2 or ZO-3 and associates with claudin, occludin, and F-actin at tight junctions, linking the actin cytoskeleton to the junction.
ZO-1 expression is reduced in many breast cancer lines.
ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions; ZO-1 is also found in cardiac myocytes.
ZO-1 may play a role in signal transduction at cell-cell junctions.
Two transcript variants encoding distinct isoforms have been identified.
Diseases associated with ZO-1 dysfunction include Celiac Disease and Congenital Nephrotic Syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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