Thermo Fisher Scientific Rabbit anti-Pig IgG (H+L) Secondary Antibody, FITC
돼지 IgG 검출용 FITC 결합 2차 항체로, 토끼에서 생산된 폴리클로날 항체입니다. Western blot, IHC, ELISA, IP에 사용 가능하며, 높은 특이성과 감도를 제공합니다. 498/517 nm 형광 특성으로 형광 분석에 적합합니다.
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- 619111
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:20–1:50 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:20–1:50 |
| ELISA | 1:20–1:50 |
| Immunoprecipitation (IP) | 1:20–1:50 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Pig |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobulin |
| Conjugate | FITC (Fluorescein Isothiocyanate) |
| Excitation/Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 40% glycerol, 1% BSA |
| Contains | 0.1% sodium azide |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2533944 |
| Target | IgG |
| Antibody Form | Whole Antibody |
Additional Formats
- HRP conjugate available
- Custom conjugation upon request
Target Information
Anti-Pig secondary antibodies are affinity-purified antibodies with well-characterized specificity for porcine (swine, pig) immunoglobulins. They are useful in detection, sorting, or purification of their specified targets.
Secondary antibodies provide versatility for various detection systems such as HRP, AP, and fluorescence, and offer enhanced sensitivity through signal amplification since multiple secondary antibodies can bind to a single primary antibody.
Typically, secondary antibodies are generated by immunizing the host animal with pooled immunoglobulins from the target species, then purified and modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to yield highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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