Thermo Fisher Scientific Calnexin Polyclonal Antibody
Rabbit polyclonal antibody targeting human Calnexin (residues 300–350). Validated for WB, IHC, IHC(P), and IP. Supplied as 1 mg/mL liquid, unconjugated form. Recommended storage at 4°C. For research use only.
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Calnexin Polyclonal Antibody
Thermo Fisher Scientific Calnexin Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Property | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 300 and 350 of human Calnexin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections
- Based on 100% sequence identity, this antibody is predicted to react with Orangutan
Target Information
Calnexin (also known as IP90, p88, and p90) is an approximately 90 kDa integral membrane protein of the endoplasmic reticulum (ER).
It functions as a molecular chaperone, assisting in the proper folding and assembly of polypeptides into multisubunit proteins.
Calnexin associates with MHC class I heavy chains, partial complexes of the T cell receptor, and B cell membrane immunoglobulin, but not with completed receptor complexes. It retains incompletely or improperly folded proteins in the ER.
Soluble ER resident proteins such as GRP78, GRP94, and protein disulfide isomerase contain a KDEL sequence for ER retention. Integral ER membrane proteins like calnexin lack KDEL but possess positively charged cytosolic residues ensuring ER localization.
Calnexin structure:
- ER luminal domain: 461 amino acids
- Transmembrane segment: 22 amino acids
- Cytoplasmic tail: 89 amino acids
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.