Thermo Fisher Scientific Calnexin Polyclonal Antibody

Thermo Fisher Scientific Calnexin Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

• Recommended shelf life: 1 year from date of receipt
• For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections
• Based on 100% sequence identity, this antibody is predicted to react with Orangutan

Target Information

Calnexin (also known as IP90, p88, and p90) is an approximately 90 kDa integral membrane protein of the endoplasmic reticulum (ER).
It functions as a molecular chaperone, assisting in the proper folding and assembly of polypeptides into multisubunit proteins.

Calnexin associates with MHC class I heavy chains, partial complexes of the T cell receptor, and B cell membrane immunoglobulin, but not with completed receptor complexes. It retains incompletely or improperly folded proteins in the ER.

Soluble ER resident proteins such as GRP78, GRP94, and protein disulfide isomerase contain a KDEL sequence for ER retention. Integral ER membrane proteins like calnexin lack KDEL but possess positively charged cytosolic residues ensuring ER localization.

Calnexin structure:

• ER luminal domain: 461 amino acids
• Transmembrane segment: 22 amino acids
• Cytoplasmic tail: 89 amino acids

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.