Thermo Fisher Scientific CD3E Recombinant Rabbit Monoclonal Antibody (BLR174J)
Thermo Fisher Scientific의 CD3E 재조합 토끼 단클론 항체(BLR174J)는 인간 및 마우스 시료에서 CD3 epsilon 단백질을 검출하는 데 사용됩니다. Western blot, IHC, ICC/IF, Flow cytometry, IP에 적합하며, 고순도 액상 형태로 제공됩니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| Flow Cytometry (Flow) | 1 µL/10⁶ cells |
| Immunoprecipitation (IP) | 12 µL/mg of lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293F |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | BLR174J |
| Immunogen | Peptide representing a region between residue 139 and the C-terminus (residue 189) of mouse T-cell surface glycoprotein CD3 epsilon |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 500 µg/mL |
| Purification | Purified |
| Storage Buffer | BBS, pH 8.2, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Immunocytochemistry: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.
Target Information
The CD3 subunit complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and regulating the cell surface expression of the TCR complex. T-cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of CD3 gamma, delta, epsilon, and zeta subunits. These are structurally related members of the immunoglobulin superfamily encoded by closely linked genes on human chromosome 11.
CD3 components have long cytoplasmic tails that associate with signal transduction molecules via a double tyrosine-based motif. CD3 may play roles in TCR-induced growth arrest, cell survival, and proliferation. The CD3 antigen is present on 68–82% of normal peripheral blood lymphocytes, 65–85% of thymocytes, and Purkinje cells in the cerebellum, but not on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. Defects in the CD3 gene are associated with CD3 immunodeficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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