Thermo Fisher Scientific TL1A Polyclonal Antibody, PeproTech

Thermo Fisher Scientific TL1A Polyclonal Antibody, PeproTech

Applications and Tested Dilution

Publications

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Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
QLRAQGEASV QFQALKGQEF APSHQQVYAP LRADGDKPRA HLTVVRQTPT QHFKNQFPAL HWEHELGLAF TKNRMNYTNK FLLIPESGDY FIYSQVTFRG MTSECSEIRQ AGRPNKPDSI TVVITKVTDS YPEPTQLLMG TKSVCEVGSN WFQPIYLGAM FSLQEGDKLM VNVSDISLVD YTKEDKTFFG AFLL.

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human TL-1A. Anti-Human TL-1A-specific antibody was purified by affinity chromatography employing an immobilized Human TL-1A matrix.

Sandwich ELISA:
To detect Human TL-1A by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human TL-1A (500-P240Bt) as a detection antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human TL-1A.

Western Blot:
To detect Human TL-1A by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used with compatible secondary reagents, the detection limit for Recombinant Human TL-1A is 1.5–3.0 ng/lane under reducing or non-reducing conditions.

Target Information

The protein encoded by this gene is a cytokine belonging to the tumor necrosis factor (TNF) ligand family. It is abundantly expressed in endothelial cells but not in B or T cells. Expression is inducible by TNF and IL-1 alpha. This cytokine acts as a ligand for receptors TNFRSF25 and TNFRSF21/DR6, activates NF-kappaB and MAP kinases, and induces apoptosis in endothelial cells. It also inhibits endothelial cell proliferation, functioning as an angiogenesis inhibitor. An additional isoform from an alternatively spliced transcript variant has been reported, but its sequence remains undetermined.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.

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