Thermo Fisher Scientific Adiponectin Polyclonal Antibody, PeproTech
인간 Adiponectin 단백질을 인식하는 Rabbit Polyclonal 항체. Western blot과 ELISA에서 사용 가능하며, 항원 친화 크로마토그래피로 정제됨. 지방 및 포도당 대사 연구에 적합. 동결 건조 형태로 제공되며 -20°C에서 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL |
| ELISA | 0.5–2.0 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Hi-5 Insect cells-derived Recombinant Human Adiponectin |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS |
| Contains | No preservative |
| Storage conditions | -20°C |
| Shipping conditions | Ambient |
| RRID | AB_2929984 |
Product Specific Information
AA Sequence of recombinant protein:
RGHHHHHHHH ETTTQGPGVL LPLPKGACTG WMAGIPGHPG HNGAPGRDGR DGTPGEKGEK GDPGLIGPKG DIGETGVPGA EGPRGFPGIQ GRKGEPGEGA YVYRSAFSVG LETYVTIPNM PIRFTKIFYN QQNHYDGSTG KFHCNIPGLY YFAYHITVYM KDVKVSLFKK DKAMLFTYDQ YQENNVDQAS GSVLLHLEVG DQVWLQVYGE GERNGLYADN DNDSTFTGFL LYHDTN.
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human Adiponectin. Anti-Human Adiponectin-specific antibody was purified by affinity chromatography employing an immobilized Human Adiponectin matrix.
Sandwich ELISA:
To detect Human Adiponectin by sandwich ELISA (using 100 µL/well), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human Adiponectin (500-P239BT) as a detection antibody, allows the detection of at least 2000–4000 pg/mL of Recombinant Human Adiponectin.
Western Blot:
To detect Human Adiponectin by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. When used with compatible secondary reagents, the detection limit for Recombinant Human Adiponectin is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
Adiponectin is a protein hormone secreted exclusively by adipocytes that regulates the metabolism of lipids and glucose. It plays a role in various physiological processes including homeostasis and obesity. Decreased levels of adiponectin are associated with obesity, insulin resistance, and hyperinsulinemia (type 2 diabetes). In mice, adiponectin has been shown to improve insulin signaling, ameliorating insulin resistance. Through activation of AMP kinase and PPAR-alpha, adiponectin increases fatty acid transport, oxidation, and dissipation in skeletal muscle, reducing intramyocellular lipid levels and improving insulin signaling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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