Thermo Fisher Scientific FceR1 alpha Monoclonal Antibody (AER-37 (CRA1)), PE-Cyanine7, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.25 µg)/test
• View 3 publications

Product Specifications

Additional Formats Available

• Unconjugated
• APC
• Biotin
• Brilliant Violet 786
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Yellow 570
• NovaFluor Yellow 610
• NovaFluor Yellow 660
• PE
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 780
• Request custom conjugation

Product Specific Information

Description:
AER-37 monoclonal antibody reacts with the Fc epsilon RI alpha subunit, an IgE-binding subunit lacking signal-transducing ability. FcεRI alpha is expressed on mast and basophil cells and is upregulated by IgE presence. It forms a tetrameric complex with beta and two gamma subunits, which possess ITAM motifs. The FcεRI complex triggers IgE-mediated allergic reactions.

Applications Reported:
Reported for use in flow cytometric analysis.

Applications Tested:
Pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. Use at 5 µL (0.25 µg) per test. A test corresponds to staining a cell sample in 100 µL final volume (10^5–10^8 cells/test).

Light Sensitivity:
Tandem dye is sensitive to photo-induced oxidation. Protect vial and stained samples from light.

Fixation:
Samples may be stored in:

• IC Fixation Buffer (Product #00-822-49)
• 1-step Fix/Lyse Solution (Product #00-5333-54)

Store up to 3 days at 4°C in the dark with minimal impact on brightness and FRET efficiency. Clone-specific performance should be verified empirically.

Excitation / Emission / Laser:
488–561 nm excitation; 775 nm emission; compatible with Blue, Green, and Yellow-Green lasers.

Filtration:
0.2 µm post-manufacturing filtered.

Target Information

FceR1 (Fc epsilonR1 alpha):
A subunit of the high-affinity IgE receptor, forming a tetrameric complex (α, β, and two γ subunits). The β and γ subunits mediate signal transduction. FcεRI plays a key role in allergic responses by coupling allergen-bound IgE to mast cells, leading to histamine release and synthesis of prostaglandins and leukotrienes—major effectors in hypersensitivity reactions such as hay fever and asthma.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

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