Thermo Fisher Scientific SIAH2 Monoclonal Antibody (24E6H3)

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-Dependent

-

Immunohistochemistry (Paraffin) (IHC (P))

1:40-1:50

-

Immunohistochemistry (Frozen) (IHC (F))

1:40-1:50

-

Immunocytochemistry (ICC/IF)

1:10

-

Flow Cytometry (Flow)

1:10

-

Product Specifications

Species Reactivity

Fruit fly, Human, Pig

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

24E6H3

Immunogen

Synthetic peptide made to an N-terminal portion of the drosophila SIAH2 protein (within residues 1-100). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:SIAH2,uniProtId:P21461-1,ncbiNodeId:7227,antigenRange:1-100,antigenLength:314,antigenImageFileName:MA5-24780_SIAH2_P21461-1_House_mouse.svg,antigenImageFileNamePDP:MA5-24780_SIAH2_P21461-1_House_mouse_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

tissue culture supernatant

Contains

0.1% sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Wet ice

RRID

AB_2717253

Product Specific Information

This is specific for human SIAH2 and Drosophila SINA in proliferating cells and stem cells. This does not detect Drosophila SINAH or normal, nondividing cells.

Target Information

SIAH-2 (seven in absentia homolog 2) is an E3 ligase that catalyzes ubiquitination and proteasome-mediated degradation of protein substrates. SIAH-2 encodes a 324 amino acid protein that shares 77% identity with human SIAH-1 and 68% identity with the Drosophila sina (seven in absentia) gene, on which development of the Drosophila R7 photoreceptor is dependent. SIAH-2 targets TRAF2 (which regulates cell responses to stress and cytokines through the regulation of key stress-signaling cascades) for degradation under stress conditions such as hypoxia. It targets HIF-1 alpha prolyl hydroxylase 3 (PHD3) for degradation upon exposure to hypoxic conditions, which coincides with an increase in SIAH-2 transcription. SIAH-2 can decrease TNF-alpha-dependent induction of JNK activity and transcriptional activation of NFkB. SIAH-2 mice subjected to hypoxia display an impaired respiratory response and reduced levels of hemoglobin.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.