Thermo Fisher Scientific BCL10 Recombinant Rabbit Monoclonal Antibody (BL10, 2988R)
BCL10 단백질을 인식하는 Thermo Fisher의 재조합 토끼 단클론 항체. IHC, ICC/IF, Flow Cytometry 등 다양한 응용에 적합. Human 시료 반응성, Protein A/G 정제, 4°C 보관. 면역 관련 신호전달 연구에 활용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | BL10, 2988R |
| Immunogen | Recombinant human BCL10 protein fragment (around aa122–168) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage conditions | 4°C |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed): Incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
BCL-10 is an N-terminal CARD (Caspase Recruitment Domain) containing protein involved in the adaptive immune response. It promotes apoptosis, pro-caspase-9 maturation, and activation of NF-kappa-B via NIK and IKK. BCL-10 acts as an adapter between the upstream TNFR1-TRADD-RIP complex and the downstream NIK-IKK-IKAP complex. It is a substrate for MALT1. Mutations in the gene can lead to Immunodeficiency 37 or mucosa-associated lymphoid type lymphoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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