Thermo Fisher Scientific Cyclin B1 (G2- and M-phase Cyclin) Monoclonal Antibody (V92.1)
Cyclin B1 단백질을 인식하는 V92.1 클론의 Mouse Monoclonal Antibody입니다. G2/M 세포주기 조절 연구에 적합하며, ICC/IF, Flow Cytometry, IP 등 다양한 응용에 사용 가능합니다. 인간, 생쥐, 햄스터 반응성을 가지며, Protein A/G로 정제된 액상 형태입니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10^6 cells |
| Immunoprecipitation (IP) | 1–2 µg/500 µg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Hamster, Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | V92.1 |
| Immunogen | Hamster Cyclin B1 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Cyclins bind to and regulate the activity of Cyclin-dependent protein kinases (CDKs). Cyclin B1 (CCNB1) is a regulatory protein involved in mitosis and is essential for controlling the cell cycle at the G2/M transition. It complexes with p34 (cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts exist: a constitutively expressed transcript and a cell cycle-regulated transcript predominantly expressed during G2/M phase. Cyclin B1 accumulates during G2 phase and is degraded at the end of mitosis via APC-dependent destruction, which is necessary for cell cycle progression. Cyclin B1 is overexpressed in various cancers, including breast, prostate, and non-small cell lung cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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