Thermo Fisher Scientific FAS Recombinant Rabbit Monoclonal Antibody (004)

Applications

Immunocytochemistry (ICC/IF)

• Tested Dilution: 1:20–1:100

Flow Cytometry (Flow)

• Tested Dilution: 1:25–1:100

Product Specifications

Additional Formats

• APC
• FITC
• PE
• Request custom conjugation

Product Specific Information

This product is preservative-free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%–0.1%).

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select optimal candidates for production.

Advantages of recombinant rabbit monoclonal antibodies:

• Better specificity and sensitivity
• Lot-to-lot consistency
• Animal origin-free formulations
• Broader immunoreactivity to diverse targets due to larger rabbit immune repertoire

This antibody has specificity for Mouse CD95/APO-1/TNFRSF6/FAS.

Target Information

FAS (Fas, CD95, APO-1) is a 46 kDa transmembrane glycoprotein that functions as a cell death receptor of the TNFR superfamily. It also exists in a soluble form (~26 kDa). Stimulation of FAS leads to aggregation of FAS-associated death domains (FADD), formation of the death-inducing signaling complex (DISC), and activation of caspases, resulting in apoptosis.

FAS can activate NF-κB, MAPK3/ERK1, MAPK8/JNK, and is involved in proliferative signaling in fibroblasts and T cells. Multiple alternatively spliced transcript variants exist, with some lacking the transmembrane domain and negatively regulating apoptosis.

The Fas/Fas ligand system plays roles in diseases such as AIDS, hepatitis, and cancer, and is involved in antiviral immune responses via induction of apoptosis.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.