Thermo Fisher Scientific Caspase 9 Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

PA5-16358 targets Caspase 9 in immunohistochemistry (paraffin), immunoprecipitation, and Western blot applications.
Shows reactivity with Bovine, Human, Mouse, Ovine, and Rat samples.
Immunogen: recombinant protein encoding aa 1–134 of human caspase 9.

Target Information

Caspase 9 (ICE-like apoptotic protease 6, ICE LAP6, apoptotic protease Mch6, apoptotic protease activating factor 3, Apaf3) is a member of the peptidase family C14 containing a CARD domain.
Caspases are cysteine proteases expressed as inactive precursors that mediate apoptosis by proteolysis of specific substrates. Caspase 9 is active as a heterotetramer, present in the cytosol, and upon activation translocates to the mitochondria.

Caspase 9 is involved in the cascade responsible for apoptosis execution and cleaves/activates Caspase 3 and Caspase 6. Binding of Caspase 9 to Apaf-1 leads to activation of the protease, which then cleaves and activates Caspase 3.
It promotes DNA damage-induced apoptosis in an ABL1/c-Abl-dependent manner and cleaves poly(ADP-ribose) polymerase (PARP).

Caspase 9 is expressed in various human tissues. An isoform of rat Caspase 9 has been identified with an alternative C-terminal sequence. Mutations in Caspase 9 affect brain development; insufficient Caspase 9 in mice results in abnormal cerebrum and neuron development.
In humans, dysfunctional Caspase 9 expression varies by tissue, and low levels may contribute to cancer and neurodegenerative diseases such as Alzheimer’s Disease (AD).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.