Thermo Fisher Scientific Phospho-ATG14 (Ser29) Polyclonal Antibody
ATG14 단백질의 Ser29 인산화 상태를 특이적으로 검출하는 폴리클로날 항체. Western blot 및 IHC에 적합하며, 인간과 생쥐 시료 반응. 고순도 정제 및 안정한 PBS/glycerol 저장 용액 제공.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:2,000
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:50–1:200
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human ATG14 (Accession Q6ZNE5), corresponding to amino acid residues around phosphorylated Ser29 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2816048 |
Product Specific Information
Antibody detects endogenous levels of Atg14 only when phosphorylated at Ser29.
Target Information
ATG14 (Autophagy related protein homolog 14) is encoded by the ATG14 gene and located on chromosome 14 in humans. Also known as ATG14L or BARKOR (Beclin 1-associated autophagy-related key regulator).
Autophagy is an evolutionarily conserved process for recycling misfolded proteins and degrading dysfunctional organelles. It is induced under nutrient stress, involving autophagosome formation, lysosomal fusion, and autophagolysosome generation.
The Class III PI3-Kinase complex (Vps34–Beclin 1) includes ATG14 (type 1) or UVRAG (type 2). Atg14 directs the type 1 complex to the preautophagosomal structure (PAS), playing a crucial role in autophagosome initiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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