Thermo Fisher Scientific KARS Polyclonal Antibody
Rabbit polyclonal antibody against human KARS protein for WB, IHC, ICC, and IP applications. Recognizes residues 550–597 of Lysyl-tRNA synthetase. Supplied as unconjugated liquid at 0.2 mg/mL. Recommended storage at 4°C; shelf life 1 year.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:100–1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| Immunoprecipitation (IP) | 1–4 µg/mg lysate |
Product Specifications
| Property | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 550 and the C-terminus (residue 597) of human Lysyl-tRNA Synthetase |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note:
- For ICC, epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE cell sections.
- For IHC, epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.
Target Information
Aminoacyl-tRNA synthetases are enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a cytoplasmic homodimer belonging to the class II family of tRNA synthetases. It is known to be a target of autoantibodies in autoimmune diseases such as polymyositis and dermatomyositis. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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