Thermo Fisher Scientific BLAP75 Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:2,000–1:10,000

Immunoprecipitation (IP)

• Tested Dilution: 2–10 µg/mg lysate

Product Specifications

Product Specific Information

• Recommended shelf life: 1 year from date of receipt
• Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Target Information

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the γ phosphate of ATP, onto an acceptor amino acid in a substrate protein. Through this mechanism, protein kinases mediate most signal transduction in eukaryotic cells, regulating metabolism, transcription, cell cycle progression, cytoskeletal rearrangement, cell movement, apoptosis, and differentiation.
The protein kinase family includes over 500 gene products and is classified into 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.
The STE group (homologs of yeast Sterile 7, 11, 20 kinases) comprises 50 kinases related to MAPK cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, including a MAPK and one or more upstream regulatory kinases (MAPKKs), are well characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate the yeast MAPK pathway.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.