Thermo Fisher Scientific MBD4 Polyclonal Antibody
Thermo Fisher Scientific의 MBD4 Polyclonal Antibody는 인간 MBD4 단백질(1-50 aa)을 항원으로 하는 토끼 유래 IgG 항체입니다. WB, IHC, IP 등 다양한 응용에 적합하며, 액상 형태로 제공됩니다. 연구용으로만 사용되며 FFPE 조직은 citrate buffer(pH 6.0)로 항원 회복을 권장합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:100–1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 1 and 50 of human methyl-CpG binding domain protein 4 (MBD4, UniProt ID: O95243-1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Target Information
DNA methylation, the addition of methyl groups to cytosine bases in CpG dinucleotides, is crucial for development and gene regulation. The methylation pattern results from enzymatic methylation and demethylation processes. MBD4, also known as MED1, is a mammalian demethylase associated with the methyl-CpG-binding domain (MBD). It catalyzes the removal of methyl groups from methylated cytosine, reverting them to cytosine.
Proteins such as MeCP2 and MBD1 repress transcription by binding to methylated DNA. MBD2 and MBD4 colocalize with heavily methylated satellite DNA, mediating methylation-related biological functions. MBD3, however, does not bind methylated DNA. MBD family members are expressed in somatic tissues, with MBD1 and MBD2 expression reduced in embryonic stem cells. MBD4 shares homology with bacterial DNA N-glycosylases/lyases and can be mutated in microsatellite unstable tumors.
For Research Use Only. Not for use in diagnostic procedures or resale without express authorization.
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