Thermo Fisher Scientific SMAD2 Polyclonal Antibody
인간, 마우스, 랫에서 반응하는 SMAD2 단백질 특이적 폴리클로날 항체입니다. Western blot, ELISA, IP에 사용 가능하며, 친화 크로마토그래피로 정제되었습니다. 액상 형태로 제공되며 -20°C에서 보관합니다. 연구용으로만 사용 가능합니다.
- 카탈로그번호
- 600-401-A59x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific SMAD2 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:3,000 |
| ELISA | 1:100,000 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to an internal region of human Smad2 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.55 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at -20°C prior to opening.
- Aliquot and freeze at -20°C or below for extended storage.
- Avoid repeated freeze/thaw cycles.
- Centrifuge if not clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
This antibody is directed against human Smad2 protein. BLAST analysis suggests cross-reactivity with Smad2 from human, mouse, and rat based on 100% sequence homology. No reactivity was detected with Smad1, Smad3, Smad4, or Smad7 in over-expressed cell lysates (Personal Communication, Kathleen Flanders, CCR-NCI, Bethesda, MD).
Target Information
SMAD2 (MADH2, MAD2) regulates multiple cellular processes such as proliferation, apoptosis, and differentiation.
It interacts with TGF-beta receptors via the SMAD anchor for receptor activation, a key step in TGF-beta signaling.
Phosphorylation of threonine 8 by ERK1 enhances SMAD2 transcriptional activity, which is negatively regulated by calmodulin.
Upon TGF-beta stimulation, SMAD2 is phosphorylated, dissociates from SARA, and associates with SMAD4 for nuclear translocation and transcriptional regulation.
SMAD2 can also mediate activin signaling and exists in alternatively spliced transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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