Thermo Fisher Scientific Calpastatin Monoclonal Antibody (SP82)
Calpastatin 단백질을 인식하는 Rabbit monoclonal antibody로, Western blot, IHC, ICC/IF, Flow cytometry에 적합합니다. Protein A/G로 정제된 액상 형태이며, 4°C 보관 필요. Calpain 억제 단백질 연구에 활용됩니다.
- 카탈로그번호
- MA516327
- 판매단위
- pk
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Thermo Fisher Scientific Calpastatin Monoclonal Antibody (SP82)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 |
| Immunocytochemistry (ICC/IF) | 1:25 |
| Flow Cytometry (Flow) | 1:100–1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SP82 |
| Immunogen | Synthetic peptide derived from near C-terminus of human calpastatin protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.6, with 1% BSA |
| Contains | 0.1% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2537846 |
Product Specific Information
Heat-mediated antigen retrieval is recommended prior to staining, using a 10 mM citrate buffer (pH 6.0) for 10 minutes, followed by cooling at room temperature for 20 minutes.
Following antigen retrieval, incubate samples with primary antibody for 30 minutes at room temperature.
A suggested positive control is breast ductal carcinoma.
Target Information
Calpastatin is an endogenous inhibitor of calpain, a calcium-dependent cysteine protease.
It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1–4).
Calpastatin is involved in proteolysis of amyloid precursor protein and in various membrane fusion events such as neural vesicle exocytosis and platelet and red-cell aggregation.
It may also influence expression levels of structural or regulatory proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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(이미지 파일: MA5-16327_Calpastatin_P20810-1_Rabbit.svg, MA5-16327_Calpastatin_P20810-1_Rabbit_PDP.jpeg)
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