
Thermo Fisher Scientific NF-H Monoclonal Antibody (NAP4)
NF-H 단백질의 인산화 형태를 특이적으로 인식하는 단클론 항체로, 신경세포 내 중간 필라멘트 연구에 적합합니다. Western blot, IHC, ICC/IF 등 다양한 응용 가능. 장기 보관 시 -20°C에서 안정적이며, ALS 및 신경퇴행성 질환 연구에 활용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:10,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:1,000 |
| Miscellaneous PubMed (Misc) | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bovine, Chicken, Horse, Human, Mouse, Pig, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | NAP4 |
| Immunogen | Native NF-H purified from bovine spinal cord |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 50% glycerol |
| Contains | 5 mM sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_568633 |
Product Specific Information
This antibody specifically recognizes the phosphorylated variant of NF-H subunit (~200–220 kDa), showing weaker reactivity with phosphorylated forms of NF-M.
Target Information
Neurofilaments are intermediate filament proteins found specifically in neurons, composed of three major proteins: NF-L, NF-M, and NF-H. NF-H is crucial for maintaining neuronal caliber and plays an important role in mature axons. It is also implicated in amyotrophic lateral sclerosis (ALS).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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