Thermo Fisher Scientific NF-H Monoclonal Antibody (NAP4)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:10,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:1,000

-

Immunocytochemistry (ICC/IF)

1:1,000

-

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Bovine, Chicken, Horse, Human, Mouse, Pig, Rat

Published species

Not Applicable

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

NAP4

Immunogen

Native NF-H purified from bovine spinal cord

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 50% glycerol

Contains

5mM sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_568633

Product Specific Information

This antibody specifically recognizes the phosphorylated variant of NF-H subunit (~200-220 kDa), showing some weaker reactivity with phosphorylated forms of NF-M.

Target Information

Involved in the maintenance of neuronal caliber, neurofilaments are the intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. NF-H has an important role in mature axons and is involved in amyotrophic lateral sclerosis (ALS).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.