Thermo Fisher Scientific MGMT Monoclonal Antibody (MT 23.2)
MGMT 단백질을 인식하는 Thermo Fisher Scientific의 MT 23.2 단클론 항체. Western blot, IHC, ICC/IF, Flow cytometry 등 다양한 응용에 사용 가능. 인간 및 마우스 반응성, Protein G 정제, 1 mg/mL 농도, 안정적인 장기 보관 가능.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | - |
| Immunohistochemistry (IHC) | - | 4 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:1,000 | 6 publications |
| Immunocytochemistry (ICC/IF) | 1:500 | - |
| Flow Cytometry (Flow) | 1–2 µg/mL | 1 publication |
| Miscellaneous PubMed (Misc) | - | 1 publication |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MT 23.2 |
| Immunogen | Recombinant human MGMT (O6-methylguanine-DNA methyltransferase) purified from E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_568616 |
Product Specific Information
- Suggested positive control: CEM-CCRF cells
- Negative control: TK6 cells
Target Information
O6-Methylguanine-DNA Methyltransferase (MGMT) is a key DNA repair protein that contributes to tumor cell resistance against alkylating chemotherapeutic agents. It protects normal cells from genotoxic and carcinogenic effects of DNA alkylation by removing alkyl groups from the O6 position of guanine. MGMT expression varies across cell types, tissues, species, and tumor groups.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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