Thermo Fisher Scientific Human BMP-7 Recombinant Protein

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

ELISA (ELISA)

Assay-dependent

-

Functional Assay (Functional)

Assay-dependent

-

Control (Ctrl)

Assay-dependent

-

Product Specifications

Species

Human, Mouse

Expression System

HEK293 cells

Molecular weight

38 kDa

Class

Recombinant

Type

Protein

Purity

>95% by SDS-PAGE

Endotoxin concentration

<0.5 EU/µg

Activity

ED50 = 8.31 ng/mL; determined by the dose-dependent induction of alkaline phosphatase production in ATDC-5 cells.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Storage buffer

10mM acetic acid

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Dry ice

Product Specific Information

Carrier-Free

Reconstitution: Centrifuge the vial briefly, before opening to bring the contents to the bottom. Reconstitute the lyophilized protein in sterile water containing 0.1% human serum albumin. Apportion the reconstituted protein into working aliquots and store at ≤ -20°C. Make any further dilutions of the reconstituted protein in low endotoxin medium or a buffered solution containing a carrier protein such as heat inactivated FCS or tissue culture grade BSA.

Storage: Store the lyophilized protein at ≤ -20°C, preferably desiccated. Upon reconstitution, apportion into working aliquots and store at ≤ -20°C. Avoid repeated freeze-thaw cycles.

Target Information

The bone morphogenetic proteins (BMPs) are a family of secreted signaling molecules that can induce ectopic bone growth. Many BMPs are part of the transforming growth factor-beta (TGFB) superfamily. BMPs were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extra skeletal site. Based on its expression early in embryogenesis, BMP7 has a proposed role in early development. In addition, the fact that BMP7 is closely related to BMP5 and BMP6 has lead to speculation of possible bone inductive activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.