Thermo Fisher Scientific Human Activin A Recombinant Protein

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

ELISA (ELISA)

Assay-dependent

-

Functional Assay (Functional)

Assay-dependent

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Control (Ctrl)

Assay-dependent

-

Product Specifications

Species

Human, Mouse

Published species

Human, Mouse

Expression System

HEK293 cells

Molecular weight

25 kDa

Class

Recombinant

Type

Protein

Purity

>95% by SDS-PAGE

Endotoxin concentration

<1 EU/mL

Activity

ED50 = 0.5 - 5 ng/mL; determined by the dose-dependent proliferation of MPC-11 cells.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Dry ice

Product Specific Information

Carrier-Free

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute lyophilized recombinant human Activin A in sterile PBS containing 0.1% human serum albumin. Further dilutions should be made in low endotoxin medium or a buffered solution containing a carrier protein such as heat inactivated FCS or tissue culture grade BSA.

Storage: Store this lyophilized preparation at ≤ -20°C, preferably desiccated. Upon reconstitution, apportion into working aliquots and store at ≤ -20°C. Avoid repeated freeze/thaw cycles.

Target Information

Activins are dimeric growth and differentiation factors which belong to the transforming growth factor-beta (TGF-beta) superfamily of structurally related signaling proteins. Activins signal through a heteromeric complex of receptor serine kinases which include at least two type I ( I and IB) and two type II (II and IIB) receptors. These receptors are all transmembrane proteins, composed of a ligand-binding extracellular domain with cysteine-rich region, a transmembrane domain, and a cytoplasmic domain with predicted serine/threonine specificity. Type I receptors are essential for signaling; and type II receptors are required for binding ligands and for expression of type I receptors. Type I and II receptors form a stable complex after ligand binding, resulting in phosphorylation of type I receptors by type II receptors. Activin A mutations are associated with fibrodysplasia ossificans progressive.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.