Thermo Fisher Scientific SPRED1 Polyclonal Antibody
인간 SPRED1 단백질을 인식하는 Rabbit Polyclonal 항체로, IHC(P)에서 1:20~1:50 희석으로 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, PBS(40% glycerol) 완충액에 0.02% sodium azide 포함. Spred1 연구 및 Ras-MAP kinase 경로 분석에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:20–1:50 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human SPRED1. Recombinant protein control fragment (Product #RP-98123). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_2647887 |
Product Specific Information
Immunogen sequence:
GQPGLDIQSR SMEYVQRQIS KECGSLKSQN RVPLKSIRHV SFQDEDEIVR INPRDILIRR YADYRHPDMW KNDLERDDAD SSI
Sequence identity with orthologs:
- Mouse: 94%
- Rat: 94%
Target Information
The Ras-MAP kinase pathway is essential for the differentiation of neuronal cells and myocytes, and it is inhibited by Spred1, a member of the Sprouty family of proteins. Spred1 acts by suppressing the phosphorylation and activation of Raf. The Spred proteins have also been implicated in the negative feedback regulation of FGF signaling in embryogenesis and angiogenesis. Expression levels of Spred1 and Spred2 proteins are inversely correlated with tumor invasion and metastasis in human hepatocellular carcinoma (HHC), suggesting potential roles as prognostic factors and therapeutic targets. Defects in this gene are associated with neurofibromatosis type 1-like syndrome (NFLS).
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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