Thermo Fisher Scientific Phospho-cdc25C (Thr48) Polyclonal Antibody
Phospho-cdc25C (Thr48) 폴리클로날 항체로 Western blot, IHC, ICC, ELISA 등 다양한 응용에 적합. Rabbit IgG 기반, Protein A 정제. Human, Mouse, Rat, Chicken 반응성. 세포 분열 조절 단백질 연구용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:200 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chicken, Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic phosphopeptide derived from human cdc25C around the phosphorylation site of Thr48 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
M-phase inducer phosphatase 3 is an enzyme encoded by the CDC25C gene in humans. This gene is highly conserved and plays a key role in regulating cell division. The encoded protein is a tyrosine phosphatase belonging to the Cdc25 phosphatase family, directing dephosphorylation of cyclin B-bound CDC2 (CDK1) and triggering entry into mitosis. It may also suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants have been described, though not all are fully characterized.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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