Thermo Fisher Scientific WIPI1 Monoclonal Antibody (OTI8F9), TrueMAB
WIPI1 단백질을 인식하는 Mouse IgG1 단클론 항체로 Western blot에 적합. Human, Mouse, Rat 반응성. 동결건조 형태로 1 mg/mL 농도, PBS buffer에 trehalose 포함. 오토파지 및 단백질 수송 연구용으로 적합.
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Applications
Western Blot (WB)
- Tested Dilution: 1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI8F9 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 195–446 of human WIPI1 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of about 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
WIPI1 (WD repeat domain, phosphoinositide interacting-1) is involved in autophagy and may regulate protein trafficking in recycling pathways.
It contains three WD repeats forming a 7-bladed propeller structure with a conserved motif that mediates protein interactions.
WIPI1 localizes to cytoplasmic vesicles, endosomes, clathrin-coated vesicles, and the trans-Golgi network.
It is ubiquitously expressed, with highest levels in heart, testis, placenta, pancreas, and skeletal muscle.
Upregulation in various tumors suggests a potential role in carcinogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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