Thermo Fisher Scientific GRP94 Monoclonal Antibody (9G10), FITC
GRP94 단백질을 인식하는 FITC 결합 단일클론 항체로, Western blot, ICC/IF, Flow Cytometry, Immunoprecipitation에 사용 가능. Rat IgG2a 아이소타입이며, 다양한 종에 반응. 498/517 nm 형광 특성으로 세포 분석에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution | Reference |
|---|---|---|
| Western Blot (WB) | 1:2,000 | - |
| Immunocytochemistry (ICC/IF) | 1:100 | - |
| Flow Cytometry (Flow) | Assay-dependent | - |
| Immunoprecipitation (IP) | Assay-dependent | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bovine, Dog, Chicken, Horse, Guinea pig, Hamster, Human, Mouse, Non-human primate, Sheep, Pig, Rabbit, Rat, Xenopus |
| Host/Isotype | Rat / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 9G10 |
| Immunogen | Purified Grp94 isolated from chicken oviducts |
| Conjugate | FITC |
| Excitation/Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage buffer | 9.1 mM sodium bicarbonate/PBS, pH 7.2, with 640.91 mM DMSO, 136.36 mM ethanolamine |
| Contains | No preservative |
| Storage conditions | 4°C, store in dark |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931551 |
Additional Conjugate Formats
Product Specific Information
0.5 µg/mL of MA5-45097 was sufficient for detection of Grp94 in 20 µg of heat-shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody.
Detects approximately 98 kDa. Does not detect human HSP90, Grp74, or GrpE from E. coli.
Target Information
GRP94 is an 803 amino acid protein belonging to the heat shock protein 90 family. It functions as a molecular chaperone involved in the processing and transport of secreted proteins.
GRP94 and its N-terminal fragment stimulate CTL expansion and maturation of human monocyte-derived dendritic cells (MDDC).
It plays a central role in both innate and acquired immunity, dendritic cell maturation and chemotaxis, antibody production, cross-priming, and acts as a potential marker in breast cancer.
It serves as a peptide acceptor in the endoplasmic reticulum and assists peptide loading of MHC class I molecules.
Expression of GRP94 suppresses A23187-induced apoptosis and stabilizes calcium homeostasis.
GRP94 is expressed in melanoma, liver metastases of colon carcinoma cells, and human gastric carcinoma BGC-823 cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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