Thermo Fisher Scientific GRP94 Monoclonal Antibody (9G10), PerCP
GRP94 단백질을 인식하는 Rat monoclonal antibody로, PerCP 형광 표지. Western blot, ICC/IF, Flow cytometry, IP 등 다양한 응용 가능. Bovine부터 Human까지 폭넓은 반응성. 단백질 G 정제, 보존제 없음, 4°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:2,000 | |
| Immunocytochemistry (ICC/IF) | 1:100 | |
| Flow Cytometry (Flow) | Assay-dependent | |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bovine, Dog, Chicken, Horse, Guinea pig, Hamster, Human, Mouse, Non-human primate, Sheep, Pig, Rabbit, Rat, Xenopus |
| Host / Isotype | Rat / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 9G10 |
| Immunogen | Purified Grp94 isolated from chicken oviducts |
| Conjugate | PerCP |
| Excitation / Emission Max | 482 / 675 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.2, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931552 |
Additional Formats
Product Specific Information
0.5 µg/mL of MA5-45098 was sufficient for detection of Grp94 in 20 µg of heat-shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody. Detects approximately 98 kDa. Does not detect human HSP90, Grp74, or GrpE from E. coli.
Target Information
GRP94 is an 803 amino acid protein belonging to the heat shock protein 90 family. It acts as a molecular chaperone involved in the processing and transport of secreted proteins. GRP94 and its N-terminal fragment stimulate CTL expansion and maturation of human monocyte-derived dendritic cells (MDDC). It plays a central role in innate and acquired immunity, dendritic cell chemotaxis, antibody production, and cross-priming. It is a potential marker in breast cancer and functions as a peptide acceptor in the endoplasmic reticulum, assisting MHC class I peptide loading. Expression of GRP94 suppresses A23187-induced apoptosis and stabilizes calcium homeostasis. GRP94 is expressed in melanoma and liver metastases of colon carcinoma, as well as in human gastric carcinoma BGC-823 cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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