Thermo Fisher Scientific TLE1 Monoclonal Antibody (OTI1H2), TrueMAB
TLE1 단백질을 인식하는 Mouse 모노클론 항체로, WB, IHC, ICC/IF, Flow Cytometry에 사용 가능. Human 시료에 반응하며, Lyophilized 형태로 제공. 고순도 Affinity chromatography 정제 및 carrier-free 형식으로 다양한 실험에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC(P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1H2 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 180–460 of human TLE1 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
TLE1 acts as a transcriptional corepressor binding various transcription factors.
It inhibits NF-kappa-B-regulated gene expression and the transcriptional activation mediated by FOXA2, CTNNB1, and TCF family members in Wnt signaling.
Full-length TLE family effects may be modulated by association with dominant-negative AES.
Also functions unusually as a coactivator for ESRRG.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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