Thermo Fisher Scientific TLE1 Monoclonal Antibody (OTI3H3), TrueMAB
TLE1 단백질을 인식하는 Mouse IgG1 단일클론 항체로, Human 시료에 반응합니다. WB, IHC, ICC/IF, Flow Cytometry에 사용 가능하며, Lyophilized 형태로 제공됩니다. 고순도 친화 크로마토그래피 정제, 안정적인 PBS buffer 구성.
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Thermo Fisher Scientific TLE1 Monoclonal Antibody (OTI3H3), TrueMAB
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3H3 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 180–460 of human TLE1 produced in E.coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Additional Formats
Product Specific Information
For reconstitution, add 100 µL distilled water to reach a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional round of desalting (Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
Transcriptional corepressor that binds to multiple transcription factors. Inhibits NF-kappa-B-regulated gene expression and transcriptional activation mediated by FOXA2, CTNNB1, and TCF family members in Wnt signaling.
Full-length TLE family members may be modulated by association with dominant-negative AES. Also functions as a coactivator for ESRRG.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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