Thermo Fisher Scientific H-Ras Monoclonal Antibody (H-Ras-03)

Applications

Western Blot (WB)

• Tested Dilution: 1–2 µg/mL
• Reducing conditions recommended

Product Specifications

Product Specific Information

This antibody reacts with human H-Ras, an ubiquitously expressed 21 kDa intracellular protein. Although reactivity with other species has not been determined, it is probable as the epitope is highly conserved among animals.

Target Information

Monomeric G protein Ras functions as a molecular switch linking receptor and nonreceptor tyrosine kinase activation to downstream signaling pathways that lead to distinct cytoplasmic or nuclear events.
Each mammalian cell contains three Ras proto-oncogenes coding for closely related Ras proteins: H-, K-, and N-Ras.
Oncogenic mutations in Ras genes are present in approximately 30% of all human cancers. Constitutive activation of Ras due to mutations or overexpression stimulates proliferation and inhibits apoptosis.

• K-Ras mutations: Common in pancreatic, colorectal, and non-small-cell lung carcinomas
• H-Ras mutations: Common in bladder, kidney, and thyroid carcinomas
• N-Ras mutations: Found in melanoma, hematological malignancies, and hepatocellular carcinoma

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.