Thermo Fisher Scientific E-cadherin Polyclonal Antibody
Rabbit polyclonal antibody recognizing human E-cadherin (CDH1) for use in WB, IHC, ICC/IF, and ELISA. Preservative-free liquid form, 1 mg/mL concentration. Suitable for cancer and cell adhesion studies. Recommended storage at 2–8 °C short-term or −20 °...
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | – |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 | – |
| Immunocytochemistry (ICC/IF) | 1:50–1:1,000 | 1 publication |
| ELISA | 1:5,000–1:10,000 | – |
Product Specifications
| Specification | Description |
|---|---|
| Published species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant Human E-Cadherin/CDH1/E-cad/CD324 protein (Met1–Ile707) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage conditions | Maintain refrigerated at 2–8 °C for up to 1 month. For long-term storage, store at −20 °C. |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2787771 |
Product Specific Information
- Preservative-free formulation.
- It is recommended to add sodium azide to avoid contamination (final concentration 0.05%–0.1%).
- Specific for Human E-Cadherin/CDH1/E-cad/CD324.
Target Information
E-Cadherin (epithelial cadherin) is a classical cadherin belonging to the calcium-dependent adhesion protein superfamily. This glycoprotein mediates cell–cell adhesion and is composed of five extracellular cadherin repeats, a transmembrane region, and a conserved cytoplasmic tail.
Mutations in the CDH1 gene are associated with gastric, breast, colorectal, thyroid, and ovarian cancers. Loss of E-cadherin function contributes to tumor progression by enhancing cell proliferation, invasion, and metastasis. The ectodomain mediates bacterial adhesion, while the cytoplasmic domain is essential for internalization.
E-cadherin plays a key role in maintaining epithelial tissue integrity and architecture. In humans, it is encoded by the CDH1 gene located on chromosome 16.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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