Thermo Fisher Scientific GRB2 Polyclonal Antibody
GRB2 단백질을 인식하는 Rabbit Polyclonal 항체로 Western blot, IHC, ICC/IF에 적합. Human, Mouse, Rat 반응성. 항원 친화 크로마토그래피로 정제된 액상 제품으로, 1 mg/mL 농도. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | - |
Product Specifications
| Category | Specification |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to a region within amino acids 156–167 of Human GRB2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2544627 |
Product Specific Information
- Recommended positive controls: 293T, A431, HeLa, HepG2, NIH-3T3, BCL-1
- Predicted reactivity: Mouse (100%), Rat (100%), Pig (100%), Chimpanzee (100%), Bovine (100%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
GRB2 protein contains one SH2 domain flanked on both sides by an SH3 domain.
In response to EGF, GRB2 binds, through its SH3 domains, to the guanine nucleotide exchange factor Sos.
The GRB2–Sos complex then binds the EGF receptor.
These interactions play a critical role in the activation of the p21 ras signaling pathway.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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