Thermo Fisher Scientific 53BP1 Polyclonal Antibody, DyLight 488
DyLight 488 형광 표지된 53BP1 폴리클로날 항체로 다양한 종에서 반응하며, WB, IHC, ICC/IF, Flow Cytometry에 사용 가능. 항원 친화 크로마토그래피로 정제되어 높은 특이성과 신뢰성 제공. 암 억제 및 DNA 손상 반응 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:10,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | Assay-Dependent |
| Immunohistochemistry (Frozen) (IHC-F) | Assay-Dependent |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| Flow Cytometry (Flow) | 1.5 µg/mL |
Product Specifications
| Category | Description |
|---|---|
| Species Reactivity | Bat, Bovine, Dog, Fish, Goat, Human, Mouse, Non-human primate, Rat |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Epitope between residues 350–400 of human tumor protein p53 binding protein 1 (NP_005648.1) |
| Conjugate | DyLight™ 488 |
| Excitation/Emission Max | 492 / 519 nm |
| Form | Liquid |
| Concentration | 0.55 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | 50 mM sodium borate |
| Contains | 0.05% sodium azide |
| Storage conditions | 4°C, store in dark |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11155998 |
Product Specific Information
- Human has been tested in WB, IHC-P, and ICC/IF.
- Mouse and goat have been tested in ICC/IF only.
- Suggested positive controls: U205, 293T, MO59K, and MEF cell lysates.
Target Information
53BP1 (P53-binding protein 1) plays a critical role in tumor suppression and acts as a substrate of ATM kinase. Upon DNA damage, 53BP1 is phosphorylated and localizes to double-strand break sites. It is involved in checkpoint signaling during mitosis, enhances TP53-mediated transcriptional activation, and contributes to genomic stability through DNA repair.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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