Thermo Fisher Scientific 53BP1 Polyclonal Antibody, Biotin
Western blot, IHC, ICC/IF 등 다양한 응용에 적합한 53BP1 폴리클로날 항체. Biotin 결합 형식으로 높은 특이성 제공. 인간, 마우스 등 여러 종에 반응. 항원 친화 크로마토그래피로 정제되어 높은 순도 보장. 연구용으로 DNA 손상 반응 연구에 유용.
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Applications and Tested Dilutions
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:200–1:10,000 | |
| Immunohistochemistry (Paraffin) (IHC-P) | Assay-dependent | |
| Immunohistochemistry (Frozen) (IHC-F) | Assay-dependent | |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 | |
| Flow Cytometry (Flow) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bat, Bovine, Dog, Human, Mouse, Rodent, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | The epitope recognized by this antibody maps to a region between residues 1925 and the C-terminus (residue 1972) of human tumor protein p53 binding protein 1 (entry NP_005648.1). |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.87 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11152498 |
Product Specific Information
- Human samples have been tested in Western Blot and ICC/IF.
- Mouse samples have been tested in ICC/IF only.
- Suggested positive control: U205, 293T, MO59K cell lysates, and MEF lysates.
Target Information
53BP1 (P53-binding protein 1) plays a critical role in tumor suppression and is a putative substrate of ATM kinase. Upon DNA damage, 53BP1 is phosphorylated and localizes to sites of double-strand breaks. It may be involved in checkpoint signaling during mitosis, enhances TP53-mediated transcriptional activation, and participates in DNA repair by maintaining genomic stability.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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