Thermo Fisher Scientific TLR4/MD-2 Complex Monoclonal Antibody (MTS510), Super Bright 436, eBioscience
마우스 TLR4/MD-2 복합체를 인식하는 MTS510 단클론 항체로, Super Bright 436 형광체가 결합되어 있습니다. Flow cytometry에 최적화되어 있으며 405 nm 레이저로 여기됩니다. 면역 반응 연구 및 TLR4 신호전달 분석에 적합합니다.
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Thermo Fisher Scientific TLR4/MD-2 Complex Monoclonal Antibody (MTS510), Super Bright 436, eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.5 µg/test | View 1 publication |
| Neutralization (Neu) | - | View 1 publication |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Not Applicable |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Super Bright™ 436 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MTS510 |
| Conjugate | Super Bright™ 436 |
| Excitation / Emission Max | 413 / 431 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2762765 |
Available Formats
- Unconjugated
- APC
- Biotin
- Functional Grade
- PE
- PE-Cyanine7
- Custom conjugation available
Product Specific Information
The MTS510 monoclonal antibody reacts with the mouse Toll-like receptor 4 (TLR4)/MD-2 complex. TLR4 is a type I transmembrane protein involved in innate immunity and inflammation, forming a complex with MD-2 and CD14 for LPS recognition and signaling.
In mice, TLR4 is expressed by thioglycolate-elicited peritoneal macrophages and is downregulated upon LPS stimulation. The TLR4 gene is defective in C3H/HeJ and C57BL/10ScCr mice, known as hyporesponders to LPS.
MTS510 co-immunoprecipitates MD-2 (~30 kDa) and TLR4 (~100 kDa), preferentially recognizing the TLR4/MD-2 complex. It has been validated for use in flow cytometric analysis of mouse bone marrow-derived dendritic cells at ≤0.5 µg/test (100 µL final volume).
Careful titration is recommended for optimal assay performance.
Super Bright 436 can be excited with a violet laser (405 nm) and emits at 436 nm. Use a 450/50 bandpass filter or equivalent.
When using multiple Super Bright dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.
Excitation: 405 nm
Emission: 436 nm
Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
MD-2 is part of the Toll/interleukin-1 receptor (TIR) family, including TLRs that recognize pathogen-associated molecular patterns (PAMPs) and mediate innate and adaptive immune responses.
TLR4, the major signaling receptor for LPS, requires MD-2 binding for maximal response. The species of MD-2 determines response specificity—for instance, human MD-2 expressed in mouse cells alters TLR4 responsiveness to LPS analogs.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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