Thermo Fisher Scientific Nitrotryptophan Monoclonal Antibody (2D12), APC

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunocytochemistry (ICC/IF)

1:50

-

Flow Cytometry (Flow)

1:100

-

ELISA (ELISA)

1:1,000

-

Product Specifications

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

2D12

Immunogen

Synthetic 6-Nitrotryptophan modified Keyhole Limpet Hemocyanin (KLH).

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

1 mg/mL

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

A 1:1,000 dilution of MA5-45817 was sufficient for detection of 6-Nitrotryptophan in 1 µg of 6-Nitrotyrptophan conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.|Specific for 6-Nitrotyrptophan modified proteins. Doss not cross-react with 7-Ketocholoesterol modified proteins.

Target Information

6-nitrotryptophan (6-NO2-Trp) is shown to be one of the most stable and major ONOO- modified amino acids, and growing evidences suggest that nitrotryptophan may play a significant role in the cellular regulation under nitrosative stress. Reactive nitrogen species (RNS) are formed in a variety of diseases such as atherosclerosis, inflammation, cancer and neurodegenerative disiases. Peroxinitrite (ONOO-) is known as a potent biological oxidant, which is formed via very fast reaction between nitric oxide (NO) and superoxide (O2-). Peroxinitrite can induce nitration of aromatic amino acids such as Tyr, Trp, Phe and His, which results in modulation of protein functions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.