Thermo Fisher Scientific Nitrotryptophan Monoclonal Antibody (2D12), APC
6-Nitrotryptophan 변형 단백질을 특이적으로 인식하는 마우스 단클론 항체. Western blot, ICC/IF, Flow cytometry, ELISA에 적합. APC 형광 표지로 651/660 nm 파장에서 검출 가능. 연구용으로 nitrosative stress 관련 단백질 분석에 활용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:50 |
| Flow Cytometry (Flow) | 1:100 |
| ELISA | 1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2D12 |
| Immunogen | Synthetic 6-Nitrotryptophan modified Keyhole Limpet Hemocyanin (KLH) |
| Conjugate | APC (Allophycocyanin) |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
A 1:1,000 dilution of MA5-45817 was sufficient for detection of 6-Nitrotryptophan in 1 µg of 6-Nitrotryptophan conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.
Specific for 6-Nitrotryptophan modified proteins. Does not cross-react with 7-Ketocholesterol modified proteins.
Target Information
6-Nitrotryptophan (6-NO2-Trp) is one of the most stable and major ONOO⁻-modified amino acids. Increasing evidence suggests that nitrotryptophan plays a significant role in cellular regulation under nitrosative stress.
Reactive nitrogen species (RNS) are formed in various diseases such as atherosclerosis, inflammation, cancer, and neurodegenerative diseases.
Peroxynitrite (ONOO⁻), a potent biological oxidant formed by the reaction between nitric oxide (NO) and superoxide (O₂⁻), can induce nitration of aromatic amino acids such as Tyr, Trp, Phe, and His, leading to modulation of protein functions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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