Thermo Fisher Scientific MDM2 Polyclonal Antibody

Applications

• Western Blot (WB): 0.3–1.0 µg/mL

Product Specifications

Product Specific Information

• Predicted to react with bovine, canine, feline, mouse, porcine, and rat based on sequence homology.
• Tested in Peptide ELISA: antibody detection limit dilution 1:64,000.

Target Information

MDM2 is a ubiquitin ligase for p53 and plays a central role in regulating p53 stability. Located on chromosome 12q, MDM2 phosphorylation by Akt at Ser166 and Ser186 enhances its interaction with p300, promoting p53 ubiquitination and degradation. Phosphorylation also prevents MDM2 binding to p19ARF, further increasing p53 degradation. Overexpression of MDM2 can inactivate p53 and reduce its tumor suppressor function. MDM2 participates in apoptosis and cell cycle regulation and is overexpressed in various human malignancies, including soft tissue carcinomas and breast cancer. It also interacts with proteins such as retinoblastoma 1 and ribosomal protein L5. More than 40 alternatively spliced transcript variants have been identified in both tumor and normal tissues.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.