Thermo Fisher Scientific CD208 (DC-LAMP) Monoclonal Antibody (31B), APC, eBioscience

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.5 µg)/test

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG2a, kappa

Recommended Isotype Control

Mouse IgG2a kappa Isotype Control (eBM2a), APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

31B

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11044500

Product Specific Information

Description: This 31B monoclonal antibody reacts with human CD208, which is also known as dendritic cell lysosome-associated membrane glycoprotein (DC-LAMP). CD208 is expressed almost exclusively by mature dendritic cells. Expression of this molecule can be induced in monocytes by stimulation with GM-CSF and IL-4. Moreover, CD208 can be upregulated in CD34+-derived dendritic cells by GM-CSF, SCF, and TNFa. CD208 is also expressed in dendritic cells of non-lymphoid tissues such as skin. While CD208 can be detected on the cell surface, this protein is mainly localized within lysosomes. CD208 plays a key role in the processing and presentation of antigen during the immune response.

The 31B antibody only recognizes intracellular CD208, not the cell surface-expressed protein.

Applications Reported: This 31B antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This 31B antibody has been pre-titrated and tested by flow cytometric analysis of in vitro differentiated mature dendritic cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Dendritic cells (DCs) are the most potent antigen-presenting cells. Immature DCs efficiently capture antigens and differentiate into interdigitating dendritic cells (IDCs) in lymphoid tissues that induce primary T-cell responses (summary by de Saint-Vis et al., 1998 [PubMed 9768752]).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.