Thermo Fisher Scientific CD205 Monoclonal Antibody (205yekta), APC, eBioscience

Thermo Fisher Scientific CD205 Monoclonal Antibody (205yekta), APC, eBioscience™

Applications and Tested Dilution

• Application: Flow Cytometry
• Tested Dilution: ≤ 0.03 µg/test
• Publications: View 2 publications

Product Specifications

Additional Formats Available

• Alexa Fluor 488
• Biotin
• NovaFluor Blue 660-120S
• NovaFluor Red 685 / 700 / 755
• NovaFluor Yellow 610 / 660 / 700 / 755
• PE / PE-Cyanine7 / PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436 / 600 / 645
• Request custom conjugation

Product Specific Information

Description:
The 205yekta antibody reacts with mouse CD205 (DEC-205), a 205 kDa integral membrane glycoprotein involved in antigen uptake, trafficking, and presentation, enhancing antigen-specific T cell immunity. CD205 is highly expressed by CD8+ dendritic cells and also found in bone marrow Gr1+ cells, Langerhans cells, bone marrow-derived dendritic cells (BMDCs), and thymic epithelial cells.

CD205 binds to PE-Cy5.5 antibody and streptavidin conjugates; therefore, these formats should not be used on CD205+ dendritic cells.

Applications Reported:
Flow cytometric analysis.

Applications Tested:
Tested on in vitro-cultured bone marrow-derived dendritic cells or mouse bone marrow cells. Recommended usage ≤ 0.03 µg/test (100 µL final volume). Cell number range: 10⁵–10⁸ cells/test. Titrate for optimal performance.

Optical Properties:

• Excitation: 633–647 nm
• Emission: 660 nm
• Laser: Red Laser

Filtration:
0.2 µm post-manufacturing filtered.

Target Information

CD205 (LY25, Lymphocyte Antigen 75) is an integral membrane glycoprotein that facilitates antigen uptake and presentation, enhancing antigen-specific T cell responses. It is highly expressed in CD8+ dendritic cells and variably expressed in bone marrow Gr1+ cells, Langerhans cells, BMDCs, and thymic epithelial cells. CD205 acts as an endocytic receptor directing captured antigens to specialized processing regions and may reduce B lymphocyte proliferation.
Dysfunction of CD205 is associated with pneumonic plague and lipid pneumonia.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.

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