Thermo Fisher Scientific TOP2A Monoclonal Antibody (UMAB145), UltraMAB
Human TOP2A 단백질을 인식하는 Mouse IgG1 단일클론 항체로, Western blot, IHC, ICC/IF 등 다양한 응용에 적합. 고순도 친화 크로마토그래피 정제 제품이며, 동결건조 형태로 제공. 연구용으로 DNA 합성 및 세포분열 관련 단백질 분석에 활용.
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Applications
Western Blot (WB)
- Assay-dependent
Immunohistochemistry (Paraffin) (IHC (P))
- Dilution: 1:200
Immunocytochemistry (ICC/IF)
- Dilution: 1:200
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | UMAB145 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1100–1531 of human TOP2A produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
Topo II alpha plays key roles in DNA synthesis, transcription, and chromosomal segregation during mitosis.
It serves as a sensitive and specific marker for late S-, G2-, and M-phases in transformed and developmentally regulated normal cells.
Topo II alpha is also implicated in tumor cell drug resistance.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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