Thermo Fisher Scientific EBP50 Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

PA1-090 detects ezrin-radixin-moesin (ERM) binding phosphoprotein of 50 kDa (EBP50) from mouse tissues, human T84 and Calu3 cells, as well as recombinant human protein.
It has been successfully used in Western blot, Immunohistochemistry, Immunofluorescence, and Immunoprecipitation.
By Western blot, this antibody detects an approximately 50 kDa protein representing EBP50 from T84 cell extract. Immunohistochemical staining of EBP50 in mouse airway results in epithelial staining.

The PA1-090 immunogen is recombinant human His-tagged EBP50 obtained from bacteria, with all His-tag specific antibodies removed.
This product was epitope affinity purified using a synthetic peptide corresponding to residues C R(286) S A S S D T S E E L N S Q D S P(302) of human EBP50.

Target Information

NHERF1 (Na+/H+ exchanger regulatory factor 1), also known as EBP50 (ezrin, radixin, moesin-binding phosphoprotein 50), is an adaptor protein that associates with beta-catenin and is required for its localization at cell-cell junctions.
It interacts with various G protein-coupled receptors and regulates their trafficking, sodium-hydrogen exchange, and sodium-dependent phosphate transport.
NHERF1/EBP50 inhibits cell motility and suppresses anchorage-independent growth. It contains a C-terminal ERM-binding region and two N-terminal PDZ domains, enabling head-to-tail intramolecular conformation to regulate interactions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.