Thermo Fisher Scientific CD170 (Siglec F) Monoclonal Antibody (1RNM44N), Brilliant Ultra Violet 737, eBioscience

Thermo Fisher Scientific CD170 (Siglec F) Monoclonal Antibody (1RNM44N), Brilliant Ultra Violet 737, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.25 µg/test

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 488, 700
• Brilliant UV 395, 563, 615
• Brilliant Violet 480, 605, 711
• eFluor 660
• NovaFluor Blue 610-70S, 660-120S, 800
• NovaFluor Red 685, 710
• NovaFluor Yellow 690, 810
• PE, PE-Cyanine7, PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436, 600, 645, 780
• Request custom conjugation available

Product Specific Information

This 1RNM44N monoclonal antibody recognizes mouse CD170 (Siglec F), a cell surface lectin in the Ig superfamily binding sialic acid-containing glycoconjugates. Siglec F is mainly expressed on eosinophils and alveolar macrophages, with lower levels on immature myelomonocytic cells. It is a functional ortholog of human Siglec 8 but not expressed on mast cells.

• Recognizes formaldehyde-fixed epitope
• Reported for flow cytometric analysis
• Tested on thioglycolate-elicited peritoneal macrophages (≤0.25 µg/test)
• Recommended titration for optimal performance
• Emission: 732 nm; Excitation: 355 nm (UV laser required)

Brilliant Ultra Violet™ 737 (BUV737) is a tandem dye sensitive to photo-induced oxidation. Protect from light during storage and staining.

Buffer Recommendations:

• Use Super Bright Complete Staining Buffer (SB-4401) or Brilliant Stain Buffer (00-4409-75) to minimize non-specific polymer interactions when using multiple polymer dyes.

Fixation Compatibility:

• Samples can be stored up to 3 days at 4°C in the dark using IC Fixation Buffer (00-8222) or 1-step Fix/Lyse Solution (00-5333).
• Compatible with short-term methanol fixation (<1 hour).

Trademark Notice:
BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

SIGLEC5 is a putative adhesion molecule mediating sialic acid-dependent binding. Expressed by monocytic/myeloid lineage cells, found in peripheral blood leukocytes, spleen, bone marrow, and at lower levels in lymph node, lung, appendix, placenta, pancreas, and thymus. Expressed by monocytes and neutrophils, absent in leukemic cell lines representing early myelomonocytic stages.

For Research Use Only. Not for use in diagnostic procedures.

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