Thermo Fisher Scientific CD163 Monoclonal Antibody (TNKUPJ), Brilliant Ultra Violet 737, eBioscience

Thermo Fisher Scientific CD163 Monoclonal Antibody (TNKUPJ), Brilliant Ultra Violet 737

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.25 µg/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 532
• APC
• Brilliant UV 615
• Brilliant UV 661
• Brilliant UV 805
• Brilliant Violet 421
• eFluor 450
• FITC
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Custom conjugation available

Product Specific Information

This TNKUPJ monoclonal antibody recognizes mouse CD163, a 130 kDa surface receptor expressed by subsets of tissue macrophages (splenic red pulp, Kupffer cells, intestinal lamina propria, and some peritoneal macrophages). Mouse monocytes do not express CD163, and it is not a good marker for M2 macrophages.

The antibody can detect CD163 on fixed and permeabilized cells, enabling intracellular staining. Aggressive tissue dissociation may reduce surface CD163; intracellular detection is recommended in those cases.

Applications Reported: Flow cytometric analysis
Applications Tested: Flow cytometry of mouse splenocytes (≤ 0.25 µg/test)

Brilliant Ultra Violet™ 737 (BUV737) emits at 732 nm and is intended for use on cytometers equipped with a 355 nm ultraviolet laser. Ensure compatibility before use.

When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize non-specific interactions.

Light Sensitivity: Protect from light to prevent photo-induced oxidation.
Fixation: Samples can be stored in IC Fixation Buffer (00-8222-49) or Fix/Lyse Solution (00-5333-54) for up to 3 days at 4°C in the dark.
Methanol Compatibility: Short-term methanol fixation is acceptable; avoid long-term methanol storage.
Excitation: 355 nm
Emission: 732 nm
Laser: Ultraviolet Laser

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

CD163 (M130 antigen, Ber-Mac3, Ki-M8, SM4) is a 130 kDa membrane glycoprotein that acts as a receptor for the hemoglobin-haptoglobin complex. It protects tissues from oxidative damage and plays a role in iron recycling. Expressed on human monocytes and macrophages in late inflammation, CD163 is upregulated by IL-10, IL-6, and dexamethasone, and shed upon LPS or PMA stimulation. Binding is calcium- and pH-dependent, with higher affinity for multimeric haptoglobin complexes. CD163 signaling induces calcium mobilization and secretion of IL6 and CSF1.

For Research Use Only. Not for diagnostic use or resale without authorization.

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