Thermo Fisher Scientific eIF3b Polyclonal Antibody
Rabbit polyclonal antibody targeting eIF3b, suitable for WB, ICC/IF, and RIP applications. Recognizes multiple species based on sequence homology. Provided as a liquid at 0.7 mg/mL concentration. Ideal for studying translation initiation and RNA-bindin...
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1 µg/mL |
| Immunocytochemistry (ICC/IF) | 5 µg/mL |
| RNA Immunoprecipitation (RIP) | 2.5 µg/10^6 cells |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide conjugated to KLH derived from within residues 150–250 of Human eIF3B |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.7 mg/mL |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_10985234 |
Product Specific Information
This antibody is predicted to react with mouse, rat, cow, and fruit fly (Drosophila melanogaster) based on sequence homology.
Target Information
RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:9388245, PubMed:17581632, PubMed:25849773, PubMed:27462815).
The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC).
It stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition, and is also required for disassembly and recycling of post-termination ribosomal complexes.
The eIF-3 complex prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation and specifically targets translation of mRNAs involved in cell proliferation, differentiation, and apoptosis.
[Source: UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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