Thermo Fisher Scientific Ets-1 Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:2,000–1:10,000

Immunoprecipitation (IP)

• Tested Dilution: 2–10 µg/mg lysate

Product Specifications

Product Specific Information

• Recommended shelf life: 1 year from date of receipt
• Based on 100% sequence identity, this antibody is predicted to react with Mouse, Rat, X. laevis, and Chicken

Target Information

ETS1 (c-Ets-1; v-ets erythroblastosis virus E26 oncogene homolog 1 (avian); ETS-1; EWSR2; p54) is a 441 amino acid protein, part of the DNA-binding ETS family.
It contains:

• N-terminal pointed domain
• Central transactivation domain
• Two autoinhibitory domains flanked by a conserved C-terminal ETS domain

ETS1 interacts with factors such as PEBP2alphaA and p300/CBP, mediating transactivation of Osteopontin, Presenilin-1, Stromelysin, and Collagenase.
Activated by Ras/MAPK signaling, its expression is upregulated in resting T-cells.
Activity is modulated by AP-1, Sp-1, c-myb, and MafB.
Sp100 activates ETS1 in nuclear bodies, while interactions with EAP1/Daxx repress MMP1 and BCL2 transactivation.
ETS1 also interacts with STAT6 to modulate Socs-1 expression induced by IL-4 and with GATA3 to positively regulate Tax-1 dependent IL-5 expression.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.